Here we provide all these functions on the platform:

    Text Searching transcripts by a keyword in gene symbol, gene description and KEGG pathway name.
    Pairwise comparison between two conditions
    Clustering Analysis
    Sets of the gene list: uploading, management, and the algebora
    Function Enrichment of GO and KEGG on a gene list Two dimention clustered heatmap
    Browsing via KEGG pathway's classical view

Forcipomyia taiwana (F. taiwana) is one of the most notorious hematophagic midges in Taiwan and Southern China. The de novo assembly of F. taiwana resulted in a genome of 130,434,311 bp assembled in 113 scaffolds. The maximum length of the scaffolds in the genome assembly is about 7.5 Mbp, and N50 is 2,601,316 bp. The genome annotation is done by MAKER3(Holt and Yandell, 2011), there are total 16987 predicted genes and 22164 predicted transcripts.

	Forcipomyia taiwana
Number of Scaffold	113
Total bases	130,434,311
Average Scaffold Length	1,154,286
Maxmum Scaffold Length	7,575,150
N50 (bp)	2,601,316
Number of MAKER3 predicted genes	16,987
Number of MAKER3 predicted transcripts	22,164

We have built-in libraries such as Virgin_males, Virgin_females, Gravid_females by our own study and NaCl_treated_eggs_24hrs(SRR13426333), NaCl_treated_eggs_24hrs(SRR13426334), Eggs_24hrs(SRR13426335), Eggs_24hrs(SRR13426336) from BMC Genomics, 3;22(1):792

Library Name	Experimental design	Source
Virgin_males	Three days post eclosion	Our study
Virgin_females	Three days post eclosion	Our study
Gravid_females	Three days post-blood-fed	Our study
NaCl_treated_eggs_24hrs(SRR13426333)	Eggs treated with 0.25 M NaCl solution and collected at 24 hrs after egg laid. (NextSeq 500)	BMC Genomics, 3;22(1):792
NaCl_treated_eggs_24hrs(SRR13426334)	Eggs treated with 0.25 M NaCl solution and collected at 24 hrs after egg laid. (Illumina HiScanSQ)	BMC Genomics, 3;22(1):792
Eggs_24hrs(SRR13426335)	Control eggs, 24 hrs after egg laid. (Illumina HiScanSQ)	BMC Genomics, 3;22(1):792
Eggs_24hrs(SRR13426336)	Control eggs, 24 hrs after egg laid. (Illumina HiScanSQ)	BMC Genomics, 3;22(1):792

Full-text search

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Search DB: blast/NR KEGG GO pFAM

Show data: SignalP tmHMM

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Pairwise Comparison

2. Apply Data Filter    Summation of expression levels by PoolA and PoolB >= <= > < =    PoolA expression level >= <= > < =   PoolB expression level >= <= > < =
3. Set the comparing scheme   fold change cutoff: >= <= > < =   expression pattern: ALL PoolA > PoolB PoolA < PoolB PoolA = PoolB
4. Select Analytic Approach:
Show Gene List
Calculate GO term enrichment      default p value cutoff 0.1 0.01 0.001 0.0001
Calculate KEGG pathway enrichment
Draw heatmap with 2D clustering (Max 3000 transcripts)
Map on Protein Network       Probability 0.4 0.5 0.6 0.7 0.8 0.9 1
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Import Genelist

1.Enter transcriptid:(one id per line)

Or upload from file:
download example

2.Select Analytic Approach:
Show Gene List (Max. 65535 transcriptid)
Calculate GO term enrichment      default p value cutoff 0.1 0.01 0.001 0.0001
Calculate KEGG pathway enrichment
Draw heatmap with 2D clustering (Max. 3000 transcriptid)
Map on Protein Network       Probability 0.4 0.5 0.6 0.7 0.8 0.9 1


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KEGG GlobalView

Gene List